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It is important always to try and prove the authenticity of the steroidal compounds of human beings. So, we used a whole animal model system which is widely used to investigate the use of human steroidal agents in relation to animal behaviour . All of our experiments were carried out with young male mice, of a normal weight and age range, on daily or fortnightly, high-fat diets for 4–8 weeks, which were also maintained (at standard temperatures and humidity) to simulate human behaviour, platinum biotech authenticity. On daily tests the animals were given 5 mg/kg in their drinking water or 0.25 mg/kg in their food, as a dietary supplement. On a fortnightly test the dose was increased to 10 mg/kg and on the same fortnight to 20 mg/kg, anabolic steroid liver damage mechanism. For the in vivo studies we used an ad libitum-feeding system, since the results were more reliable if a group received only one, rather than a mixture of a diet and the supplement. We also tested the behaviour of female mice at the same dose as those females were receiving in their home cages and the behaviour of male mice and their offspring was studied in situ in the test cages. We used the open field and social testing paradigm, platinum biotech authenticity. The open field test was a method that has recently been used to investigate stress-induced memory  by exposing groups of animals to a stressful event within a specially designed enclosed arena with four fixed corners each. The object was presented in the centre so that it was exposed to all stimuli before the open field test began  ; in this way, it would be obvious what kind of object would be presented to your favourite animal as long as you kept an eye on it, anabolic steroid nandrolone in pork. We compared the degree of stress-induced memory in the treated and non-treated animals by comparing the rate of occurrence of fear and the number of trials per session, i.e. number of trials spent in the centre of the arena. The number of trials were measured in a randomised and double-blind order by two independent observers, and were recorded in the control cages. The animal that spent the most time at the centre of the arena had the greatest number of trials per session, indicating the presence of stress, whereas those animals, which spent most time in the centre displayed the least amount of fear from the event, anabolic steroid legal countries. This study was carried out in compliance to the guidelines given by the Animal Experiments Committee (AEC) of the UK National Institute for Health and Clinical Excellence (NICE)  which is the UK government clinical standard for animal research in all categories.