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Bca protein assay
A protein assay can be set up to measure the concentration of the unknown protein sample (mg/ml), or it can be set up to determine the total amount of protein in the unknown protein sample (mg). The BCA assay has a linear concentration range between 200–1,000 µg of protein per milliliter. The BCA protein assay is based on a biuretreaction, which is the reduction of Cu 2+ toCu + by proteins in an alkaline solution withconcentration-dependent detection of themonovalent copper ions. # 786 -570 is for 500 x 1ml assays or 2,500 x Micro -well. Scans of eight BSA standards (0 to 2000 µg/mL) tested with the Thermo Scientific Pierce BCA Protein Assay Kit (Part No. The thick line is the 2000 µg/mL sample.
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Thermo Scientific ® Pierce BCA Protein Assays are one of the great workhorses for measuring the protein concentration of a sample. One of the best features of the Pierce ® BCA Protein Assay method is its compatibility with many detergents at. Acid (BCA) assay [4], and the colloidal gold protein assay [5]. The most common protein assay reagents involve either protein–dye binding chemistry (Coomassie/Bradford) or protein–copper chelation chemistry (biuret/BCA). A protein assay can be set up to measure the concentration of the unknown protein sample (mg/ml), or it can be set up to determine the total amount of protein in the unknown protein sample (mg). The BCA assay has a linear concentration range between 200–1,000 µg of protein per milliliter. # 786 -570 is for 500 x 1ml assays or 2,500 x Micro -well. The fewer compounds you can use to get to your goals, the better. The easier it is for you to understand how your body reacts to these compounds. 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